Revvity Alphalisa Surefire Ultra Human And Mouse Total Irf5 Detection Kit, Control Lysate

Revvity this control lysate is intended for use with the alphalisa surefire ultra human and mouse total irf5 detection kit or for use as a control for those running orthogonal or secondary assays along with the surefire assay. to prepare the lysate, thp-1 cells were seeded at 300k/mL, and incubated for 48 hours in t175 flasks in 10% FBS containing medium. cells were harvested and resuspended at 8 x 106 cells/mL and treated with 100 nm calyculin a for 3 hours. cells were then washed in HBSS + 0.1% BSA and lysed in lysis buffer at a final cell density of 8 x 106 cells/mL. - PEMSD (Additional S&H Or Hazmat Fees May Apply)