Revvity FFA120 Assay Reagent Kit

Kit contains:

• FFA120 Ladder (CLS158127, 1 vial, storage temperature -25 to -15°C)
• FFA chip storage buffer (5 vials, storage temperature 2-8°C)
• FFA marker (1 vial, storage temperature 2-8°C)
• FFA gel matrix (6 vials, storage temperature 2-8°C)

Key Specification:

• Sizing range: 20-120 nt
• Concentration range: 4 nM – 2.5 µM Cy5-labeled DNA/RNA fragment
• Sensitivity: 4 nM Cy5-labeled DNA/RNA fragment (before DMSO dilution)
• Sizing resolution: 5 nt
• Sizing reproducibility: CV < 5.0% or 4 nt
• Run time: 60 seconds per well (~2.5 hours for 96 wells)
• Chip preps for reagent kit: 5 HT preps or 10 LT preps
• Maximum salt concentration: ≤ 20 mM Tris, 100mM KCl
• Sample volume requirement: > 2 µL

Plates, Chips and Reagents sold separately:

• HT DNA 5K/HT RNA LabChip^® Assay (LabChip^® GX Touch^™ HT, 760435)
• 24 DNA 5K/HT RNA LabChip^® Assay (LabChip^® GX Touch^™ 24, CLS138949)
• Sipper wash solution (CLS158129)

Assay run program available from download:

• FFA120. asyx LabChip^® GX Touch™ Software v1.10 or higher and LabChip^® GX Reviewer v5.8 or higher are required.

Citation:

Sun, Y. et al. (2023) ‘Application of microfluidic chip electrophoresis for high-throughput nucleic acid fluorescence fragment analysis assays’, NAR Genomics and Bioinformatics, 5(1). doi:10.1093/nargab/lqad011.