Revvity PhenoVue 503 Lysosomal stain

Revvity Lysosomes are abundant organelles which contain more than sixty acidic enzymes. Lysosomes play essential role in macromolecules and organelles break down through the autophagy-lysosomal system. Defective regulation of lysosomal function has been reported in several diseases such as lysosomal storage disorders and neurodegenerative diseases like Alzheimer’s and Parkinson’s. PhenoVue 503 lysosomal stain is partially protonated at neutral pH, and highly protonated at acidic pH. In living cells, PhenoVue 503 lysosomal stain accumulates in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining. PhenoVue 503 lysosomal stain can be used to lysosomes in immunofluorescence, as well as high-content analysis and screening applications. Features Numbers of Vials Per Unit 20 Quantity or Volume Per Unit 18.1µg (50nmoles) Form Solution 1mM DMSO Storage -16°C Recommended Working Concentration 50 ng/mL Maximum Excitation Wavelength 503 nm Maximum Emission Wavelength 510 nm Common Filter Set FITC Live Cell Staining Yes Fixed Cell Staining No Equivalent Number of Microplates 650 - 2050 x 96-well microplates 550 - 2050 x 384-well microplates 1050 - 3250 x 1536-well microplates - PEMSD