Revvity Aequozen Human Orexin Ox<Sub>1</Sub> Cell Line, CHO-K1 Frozen Cells

Frozen Cells validated for calcium testing: Orexin OC₁ receptor, human recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.

Our AequoZen growth arrested cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.

Features:

• Pre-validated batches of ready-to-use cells for calcium testing
• Optimized to alleviate biological variability
• Step-by-Step protocols for culture-free functional testing on our validated cells
• Affordable access to small aliquots of cells (minimum size: 10 million cells/vial)
• Ready-to-use cells for Profiling, Pharmacology or Screening applications
• Up-scaling and customized developments

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance.