Revvity Depletex Rna Depletion (Globin)

Enhance RNA-Seq quality by eliminating globin fragments. Apart from ribodepletion, RNA samples often contain a limited number of well-characterized, highly expressed transcripts. However, resequencing these transcripts contributes minimally to biologically relevant information. The dominance of highly expressed transcripts hinders coverage for low-expressing counterparts, resulting in reduced sensitivity for detecting disease-related low-expressing genes, rare variants, and unique fusion events. Globin mRNA dominates the mRNA content of red blood cells and whole blood. Three globlin genes (HBA1, HBA2 and HBB) constitute ≥30% of total mRNA from blood. Key highlights: Unbiased removal of human globin from RNA-seq libraries Achieve >99% depletion of contaminating globin Rapid one-hour depletion incubation