Revvity Nextflex Rapid Directional Rna-Seq Kit 2.0

• High coverage uniformity with low duplication rate
• Optimized for use with 5 ng – 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included
• Simple protocol validated with NEXTFLEX^® poly(A) beads 2.0 and NEXTFLEX^® RiboNaut^™ rRNA depletion kit (human, mouse, rat)
• Designed to work with NEXTFLEX^® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 1,526 samples in one run)
• Automated on the Sciclone^® G3 NGSx and Zephyr^® G3 NGS workstations

The NEXTFLEX^® Rapid Directional RNA-seq kit 2.0 includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been tested with the NEXTFLEX^® poly(A) beads 2.0 and NEXTFLEX^® RiboNaut^™ rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX^® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX^® RNA-Seq 2.0 Unique Dual Index Barcodes (6.25 μM), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample.