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CamelidVHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx)is a peptide chain about 110 amino acids in length, containing one variable domainof a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelidVHH antibody is able to bind selectively to a specific antigen. This single-domainantibody has a broad range of applications in biotechnical and therapeutic researchfields due to its small size, simple production and high affinity. GenScript’s MonoRab™ Anti-Camelid VHHAffinity Resin (Cat. No. L00905) is designed for purification of Camelid VHHsingle domain antibody from commonly used protein expression systems such as bacteria,yeast and mammalian cells. The VHH single domain antibody in cell lysate can specificallybind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecificbinding reactions are eliminated by stringent washing steps, and the purified CamelidVHH single domain antibody is eluted with a high recovery rate. Table1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHHAffinity Resin.
Table 1. Characteristics of Anti-Camelid VHH AffinityResin
Productcontent
50%settled resin in TBS with 0.02%sodium azide
Matrix
4% cross-linkedagarose
Averagebead size
90 μm
Ligand
MonoRab™Rabbit Anti-Camelid VHH Antibody, mAb
Bindingcapacity
Approximately2-4 mg Camelid VHH protein per mLsettled resin (depending on amino acid sequence of different Camelid VHHs)
Storageand stability
Storeat 2-8 °C for up to 12 months. Do not freeze the resin.
Resinreuse
Whenstored at 2 - 8° C, the resin can be recycled at least 5 timeswith no loss of binding capability. The resin can be reused up to 10 times withminimum loss of binding capability.
Elutionmethod
Acidbuffer elution (pH 2.5)
Reagentscompatibility
Compatiblewith commonly used bacterial lysis reagents. For reagents compatible withpurification, see tables 2 and 3 in the manual.
Note
Limited Use Label license: This product may be the subject of one or more patents filed by GenScript Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer should not sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. For commercial use, please contact GenScript at product@genscript.com.