IBI Scientific Maxi Fast-Ion Plasmid Kit, 1 to 1.5 ug Yield

IBI Scientific Maxi Fast-Ion Plasmid Kit, 1.5 ug Binding Capacity, 1 to 1.5 ug, 200 to 800 mL High-Copy, 500 to 1600 mL Low-Copy Culture Input, Anion-Exchange Format, <180 min Processing Time, 1 to 20 kb Fragment Size, 500 uL Elution Volume to 2 mL Elution Volume, 15 to 25 deg C Storage Tempature. The maxi plasmid kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hr. The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR and in vitro transcription. The quality of the Fast-Ion Maxi Kits is tested on a lot-to-lot basis by isolating plasmid DNA from a 100 mL overnight E. coli (DH5α) culture, containing pBluescript (A600>2 U/mL). More than 400 ug of plasmid DNA is quanti-fied with a spectrophotometer. The purified plasmid (1 ug) is used in EcoRI digestion and checked by electrophoresis - IBI (Additional Shipping And Hazmat May Apply)