Cell Signaling Avi-Tag Antibody

Name: Cell Signaling Avi-Tag Antibody
SKU: CSIG-10643S
Price: 317.14 USD

List Price $317.14 Your Price $317.14

Cell Signaling Avi-Tag Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-10643S

MFG.PART: 10643S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	All
Sensitivity	Transfected Only
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Avi-Tag Antibody detects exogenously expressed proteins containing the Avi epitope tag. This antibody recognizes the Avi tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Avi-Tag Antibody detects exogenously expressed avi-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.
Species Reactivity	All Species Expected
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide containing the Avi sequence (GLNDIFEAQKIEWHE). Antibodies are purified by peptide affinity chromatography.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.Avi-Tag is a small artificial peptide tag specifically recognized and biotinylated by the E. coli biotin ligase BirA (1). Biotinylated avi-tagged proteins can be detected or purified by streptavidin or avidin, exploiting their strong affinity for biotin (1,2).