Cell Signaling Gatad2a (E7b5b) Rabbit mAb

Name: Cell Signaling Gatad2a (E7b5b) Rabbit mAb
SKU: CSIG-17705S
Price: 354.51 USD

List Price $354.51 Your Price $354.51

Cell Signaling Gatad2a (E7b5b) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-17705S

MFG.PART: 17705S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	70, 73
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Chromatin IP: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GATAD2A (E7B5B) Rabbit mAb recognizes endogenous levels of total GATAD2A protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro627 of human GATAD2A protein.
Background	GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2A interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2A at Lys30 and Lys487 (2-5). In addition to being sumoylated, GATAD2A has also been shown to be phosphorylated by AMPK in the memory formation process (6). Depletion of GATAD2A in order to reduce NuRD-dependent repression of key pluripotency genes is being explored in the context of induced pluripotent stem cell (iPSC) generation (7,8).