Cell Signaling Mbp Tag (8g1) Mouse mAb

Name: Cell Signaling Mbp Tag (8g1) Mouse mAb
SKU: CSIG-2396S
Price: 348.45 USD

List Price $348.45 Your Price $348.45

Cell Signaling Mbp Tag (8g1) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2396S

MFG.PART: 2396S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	All
Sensitivity	Transfected Only
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200, Immunofluorescence (Immunocytochemistry): 1:1600}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	MBP Tag (8G1) Mouse mAb detects recombinant proteins fused to maltose binding protein (MBP).
Species Reactivity	All Species Expected
Source/Purification	Monoclonal antibody is produced by immunizing animals with an MBP fusion protein.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.One commonly used system is the maltose-binding protein (MBP) (1). In this system, the target gene is inserted in the appropriate vector and expressed. Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein (2). An antibody to the MBP tag can also be used to isolate or detect expression of the protein.

Specifications