Cell Signaling Eif4a (F52) Antibody

Name: Cell Signaling Eif4a (F52) Antibody
SKU: CSIG-2425S
Price: 320.17 USD

List Price $320.17 Your Price $320.17

Cell Signaling Eif4a (F52) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2425S

MFG.PART: 2425S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	48
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	eIF4A (F52) Antibody detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe52 of human eIF4A. Antibodies are purified by protein A and peptide affinity chromatography.
Background	A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5 m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5 mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).

Specifications