Cell Signaling Shc Antibody

Name: Cell Signaling Shc Antibody
SKU: CSIG-2432S
Price: 320.17 USD

List Price $320.17 Your Price $320.17

Cell Signaling Shc Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2432S

MFG.PART: 2432S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	46, 52, 66
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Shc Antibody detects endogenous levels of total Shc protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy-terminal PTB domain of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.
Background	SHC-transforming protein 1 (SHC1) is an adaptor protein to targets of activated tyrosine kinases. SHC1 is ubiquitously expressed and the gene is expressed in 2 transcripts, resulting in 3 isoforms with distinct functions and subcellular locations (1). The phosphorylated p46 and p52 isoforms couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex. The third isoform, p66, acts as a downstream target of the tumor suppressor p53 and is thereby implicated in oxidative stress response, tumor progression, and cancer cell proliferation (2,3). SHC1 has been identified to have prognostic and diagnostic value in multiple cancers, making it a potential biomarker (4).