Cell Signaling Gfp Antibody

Name: Cell Signaling Gfp Antibody
SKU: CSIG-2555S
Price: 317.14 USD

List Price $317.14 Your Price $317.14

Cell Signaling Gfp Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2555S

MFG.PART: 2555S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	All
Sensitivity	Transfected Only
Molecular Weight (kDa)	27
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:1250 - 1:5000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GFP Antibody detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein.
Species Reactivity	All Species Expected
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic GFP peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1).

Specifications