Cell Signaling Phospho-Prk1 (Thr774)/Prk2 (Thr816) Antibody

Name: Cell Signaling Phospho-Prk1 (Thr774)/Prk2 (Thr816) Antibody
SKU: CSIG-2611S
Price: 365.62 USD

List Price $365.62 Your Price $365.62

Cell Signaling Phospho-Prk1 (Thr774)/Prk2 (Thr816) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2611S

MFG.PART: 2611S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	120 Phospho-PRK1. 140 Phospho-PRK2.
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-PRK1 (Thr774)/ PRK2 (Thr816) Antibody detects endogenous levels of PRK1 and PRK2 only when phosphorylated at Thr774 or Thr816, respectively. This antibody also detects PKC zeta and lambda when phosphorylated at Thr 410 and 403, respectively.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr774 of human PRK1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

Specifications