Cell Signaling Lck (L22b1) Mouse mAb

Name: Cell Signaling Lck (L22b1) Mouse mAb
SKU: CSIG-2657S
Price: 348.45 USD

List Price $348.45 Your Price $348.45

Cell Signaling Lck (L22b1) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2657S

MFG.PART: 2657S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	56
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Lck (L22B1) Mouse mAb detects endogenous levels of total Lck protein. This antibody does not cross-react with other Src family members.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a GST-Lck fusion protein containing amino acid residues 1 to 234 of human Lck.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).