Cell Signaling Eif6 Antibody

Name: Cell Signaling Eif6 Antibody
SKU: CSIG-3263S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Eif6 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3263S

MFG.PART: 3263S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	27
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:50, Immunofluorescence (Immunocytochemistry): 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	eIF6 Antibody detects endogenous levels of total eIF6 protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eIF6. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3).

Specifications