Cell Signaling Perilipin-1 (D418) Antibody

Name: Cell Signaling Perilipin-1 (D418) Antibody
SKU: CSIG-3470S
Price: 381.78 USD

List Price $381.78 Your Price $381.78

Cell Signaling Perilipin-1 (D418) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3470S

MFG.PART: 3470S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	62
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:100, Immunofluorescence (Immunocytochemistry): 1:200}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Perilipin-1 (D418) Antibody detects endogenous levels of total perilipin-1 protein.
Species Reactivity	Human, Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Asp418 of human perilipin-1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5).

Specifications