Cell Signaling Nanog Antibody

Name: Cell Signaling Nanog Antibody
SKU: CSIG-3580S
Price: 317.14 USD

List Price $317.14 Your Price $317.14

Cell Signaling Nanog Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3580S

MFG.PART: 3580S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	42
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:800, Flow Cytometry (Fixed/Permeabilized): 1:400, Chromatin IP: 1:25}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Nanog Antibody detects endogenous levels of total nanog protein.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence at the N-terminus of human nanog. Antibodies are purified by Protein A and peptide affinity chromatography.
Background	Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

Specifications