Cell Signaling Phospho-Ikkalpha/Beta (Ser176/180) (16a6) Rabbit mAb (BSA And Azide Free)

Name: Cell Signaling Phospho-Ikkalpha/Beta (Ser176/180) (16a6) Rabbit mAb (BSA And Azide Free)
SKU: CSIG-36214SF
Price: 906.98 USD

List Price $906.98 Your Price $906.98

Cell Signaling Phospho-Ikkalpha/Beta (Ser176/180) (16a6) Rabbit mAb (BSA And Azide Free) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-36214SF

MFG.PART: 36214SF

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µg
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	85 IKK-alpha 87 IKK-beta
Source/Isotype	Rabbit IgG
Application/Dilution	{}
Storage	Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #2697
Specificity/Sensitivity	Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb (BSA and Azide Free) detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα.
Background	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do SO through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).