Cell Signaling P27 Kip1 (D37h1) Rabbit mAb

Name: Cell Signaling P27 Kip1 (D37h1) Rabbit mAb
SKU: CSIG-3688S
Price: 351.48 USD

List Price $351.48 Your Price $351.48

Cell Signaling P27 Kip1 (D37h1) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3688S

MFG.PART: 3688S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	27
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	p27 Kip1 (D37H1) Rabbit mAb detects endogenous levels of total p27 Kip1 protein. The antibody may cross-react with p57 Kip2.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p27 Kip1.
Background	p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).