Cell Signaling Grb10 Antibody

Name: Cell Signaling Grb10 Antibody
SKU: CSIG-3702S
Price: 320.17 USD

List Price $320.17 Your Price $320.17

Cell Signaling Grb10 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3702S

MFG.PART: 3702S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	76
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GRB10 Antibody detects endogenous levels of isoform 1 of total GRB10 protein independent of phosphorylation. The antibody does not cross-react with GRB7 or GRB14.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human GRB10. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by pH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4).