Cell Signaling Beta-Catenin (15b8) Mouse mAb

Name: Cell Signaling Beta-Catenin (15b8) Mouse mAb
SKU: CSIG-37447S
Price: 353.50 USD

List Price $353.50 Your Price $353.50

Cell Signaling Beta-Catenin (15b8) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-37447S

MFG.PART: 37447S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	92
Source/Isotype	Mouse IgG1 kappa
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:400 - 1:1600, Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400}
Storage	Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.
Specificity/Sensitivity	β-Catenin (15B8) Mouse mAb recognizes endogenous levels of total β-Catenin protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a recombinant chicken β-Catenin protein.
Background	β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Specifications