Cell Signaling Bak Antibody

Name: Cell Signaling Bak Antibody
SKU: CSIG-3814S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Bak Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3814S

MFG.PART: 3814S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	25
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Bak Antibody detects endogenous levels of Bak protein. No cross reactivity was detected with other Bcl-2 family members.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly82 of human Bak. Antibodies were purified by protein A and peptide affinity chromatography.
Background	Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).

Specifications