Cell Signaling Phospho-Pld1 (Thr147) Antibody

Name: Cell Signaling Phospho-Pld1 (Thr147) Antibody
SKU: CSIG-3831S
Price: 365.62 USD

List Price $365.62 Your Price $365.62

Cell Signaling Phospho-Pld1 (Thr147) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3831S

MFG.PART: 3831S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	120
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-PLD1 (Thr147) Antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147. This antibody does not cross-react with phosphorylated PLD2.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr147 of human PLD1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified SO far. Both are regulated by protein kinases, small GTPases and Ca2+ (1). PLD1 is phosphorylated at Ser2, Ser561, and Thr147 by PKC (2,3). Phosphorylation at Thr147 and Ser561 regulates PLD1 activity (3).