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Cell Signaling Il-2ralpha/Cd25 (E9w2j) Xp Rabbit mAb
List Price $178.77 Your Price $178.77
Cell Signaling Il-2ralpha/Cd25 (E9w2j) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-39475T
MFG.PART: 39475T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 40-80 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, IHC Leica Bond: 1:50 - 1:200, Immunohistochemistry (Paraffin): 1:50 - 1:200, Immunofluorescence (Frozen): 1:800, Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400, Flow Cytometry (Live): 1:100 - 1:400} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product #95742. |
| Specificity/Sensitivity | IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb recognizes endogenous levels of total IL-2Rα/CD25 protein. This antibody non-specifically labels myenteric nerves in the gut. |
| Species Reactivity | Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse IL-2Rα/CD25 protein. |
| Background | Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). |
| SKU | CSIG-39475T |
|---|---|
| Supplier Part Number | 39475T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |