Cell Signaling Thex1 Antibody

Name: Cell Signaling Thex1 Antibody
SKU: CSIG-4049S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Thex1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4049S

MFG.PART: 4049S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	M R
Sensitivity	Endogenous
Molecular Weight (kDa)	42
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	THEX1 Antibody detects endogenous levels of total THEX1 protein.
Species Reactivity	Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse THEX1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	THEX1 (3’hExo) is a 3’ exonuclease that may play a role in the degradation of histone mRNA transcripts (1). A recently identified member of the DEDDh 3 exonuclease family, THEX1 binds the conserved stem-loop structure found at the 3’ end of mRNA in vitro (2). The binding of THEX1 to mRNA requires the presence of a terminal ACCCA sequence and is enhanced by the concurrent binding of stem-loop binding protein (SLBP). Cleavage of histone mRNA by THEX1 exonuclease may help produce the rapid turnover of histone mRNA transcripts associated with the completion of DNA replication (3). Additional evidence suggests that THEX1 may be responsible for excising the remaining few 3’ nucleotides following cleavage by a different enzyme (4).

Specifications