Cell Signaling Gadd34 (E3s6n) Rabbit mAb

Name: Cell Signaling Gadd34 (E3s6n) Rabbit mAb
SKU: CSIG-41222S
Price: 354.51 USD

List Price $354.51 Your Price $354.51

Cell Signaling Gadd34 (E3s6n) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-41222S

MFG.PART: 41222S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	110
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GADD34 (E3S6N) Rabbit mAb recognizes endogenous levels of total GADD34 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala69 of human GADD34 protein.
Background	The growth arrest and DNA damage-inducible protein, GADD34, is essential for cell stress responses. It is induced by stresses such as DNA damage and endoplasmic reticulum (ER) stress. ER stress stimulates the unfolded protein response (UPR), increasing phosphorylation of Ser51 in the α subunit of eukaryotic translation initiation factor 2 (eIF2α). This change represses translation. Phosphorylation of eIF2α also increases GADD34 expression. GADD34 binds to type 1 protein phosphatase 1 (PP1), a serine/threonine phosphatase, and promotes PP1 to dephosphorylate eIF2α (1,2). GADD34 expression is correlated with eIF2α dephosphorylation late in the stress response, suggesting a critical role of GADD34 in a negative feedback loop to reduce stress response signaling and shift cells from protein synthesis inhibition to stress-induced gene expression (3).