Cell Signaling Phospho-Hsl (Ser563) Antibody

Name: Cell Signaling Phospho-Hsl (Ser563) Antibody
SKU: CSIG-4139T
Price: 153.52 USD

List Price $153.52 Your Price $153.52

Cell Signaling Phospho-Hsl (Ser563) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4139T

MFG.PART: 4139T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	M
Sensitivity	Endogenous
Molecular Weight (kDa)	81, 83
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-HSL (Ser563) Antibody detects endogenous levels of HSL only when phosphorylated at Ser563 by PKA. This antibody does not cross-react with Ser565 phosphorylated HSL.
Species Reactivity	Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human HSL (equivalent to Ser563 of rat HSL). Antibodies are purified by protein A and peptide affinity chromatography.
Background	HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).