Cell Signaling Phospho-Mkk7 (Ser271/Thr275) Antibody

Name: Cell Signaling Phospho-Mkk7 (Ser271/Thr275) Antibody
SKU: CSIG-4171S
Price: 365.62 USD

List Price $365.62 Your Price $365.62

Cell Signaling Phospho-Mkk7 (Ser271/Thr275) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4171S

MFG.PART: 4171S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	48
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-MKK7 (Ser171/Thr275) Antibody detects endogenous levels of MKK7 phosphorylated at serine 271 and threonine 275. This antibody does not cross-react with phosphorylated SEK1/MKK4 or other phosphorylated MAP kinase kinases.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser271/Thr275 of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.
Background	MKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK/JNK pathway (1,2). MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

Specifications