Cell Signaling Afp (D12c1) Rabbit mAb

Name: Cell Signaling Afp (D12c1) Rabbit mAb
SKU: CSIG-4448S
Price: 420.16 USD

List Price $420.16 Your Price $420.16

Cell Signaling Afp (D12c1) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4448S

MFG.PART: 4448S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	65
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	AFP (D12C1) Rabbit mAb recognizes endogenous levels of total AFP protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AFP protein.
Background	Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).