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Cell Signaling Lysyl Hydroxylase 2 Antibody
List Price $320.17 Your Price $320.17
Cell Signaling Lysyl Hydroxylase 2 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-44709S
MFG.PART: 44709S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 100 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Lysyl Hydroxylase 2 Antibody recognizes endogenous levels of total Lysyl Hydroxylase 2 protein. |
| Species Reactivity | Human |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Lysyl Hydroxylase 2 protein. Antibodies are purified by peptide affinity chromatography. |
| Background | The Extracellular Matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules, including proteins, glycoproteins, proteoglycans, and polysaccharides (1,2). One of the major proteins that comprises the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple-helix structure that is characteristic of all collagens (3). The large family of collagens is divided into three subgroups: fibrillar collagens, non-fibril forming collagens, and fibril-associated collagens. These subgroups differ in their structure and supramolecular assembly (3).Collagen content and abundance determines the nature of the scaffold of the ECM, and is known to influence pathological events such as tumor growth and invasion, and also tissue fibrosis (1). Lysyl Hydroxylase 2 (encoded by the PLOD2 gene), is an important enzyme that mediates the hydroxylation of lysines in collagen-like peptides in the ER, a key step that contributes to cross-linking of collagen fibrils (4). Interestingly, overexpression of Lysyl Hydroxylase 2 observed in multiple cancers, driven potentially through hypoxia-induced mechanisms, is related to a poor prognosis, tumor migration, invasion, and EMT (5,6). Current interest exists in pharmaceutical inhibition of this enzyme for therapeutic intervention to prevent metastasis and tumor spread (6). |
| SKU | CSIG-44709S |
|---|---|
| Supplier Part Number | 44709S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |