Cell Signaling Mouse Anti-Rabbit Igg (Light-Chain Specific) (D4w3e) mAb

Name: Cell Signaling Mouse Anti-Rabbit Igg (Light-Chain Specific) (D4w3e) mAb
SKU: CSIG-45262S
Price: 252.50 USD

List Price $252.50 Your Price $252.50

Cell Signaling Mouse Anti-Rabbit Igg (Light-Chain Specific) (D4w3e) mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-45262S

MFG.PART: 45262S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	Rab
Source/Isotype	Mouse
Application/Dilution	{}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.
Species Reactivity	Rabbit
Source/Purification	Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Background	Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb as a secondary antibody will eliminate this problem.