Cell Signaling Ikappabalpha (L35a5) Mouse mAb (Amino-Terminal Antigen) (Apc Conjugate)

Name: Cell Signaling Ikappabalpha (L35a5) Mouse mAb (Amino-Terminal Antigen) (Apc Conjugate)
SKU: CSIG-46001S
Price: 469.65 USD

List Price $469.65 Your Price $469.65

Cell Signaling Ikappabalpha (L35a5) Mouse mAb (Amino-Terminal Antigen) (Apc Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-46001S

MFG.PART: 46001S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M R Mk B Pg
Sensitivity	Endogenous
Source/Isotype	Mouse IgG1
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (APC Conjugate) detects endogenous levels of total IκBα protein.
Species Reactivity	Human, Mouse, Rat, Monkey, Bovine, Pig
Source/Purification	Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding to the amino terminus of human IκBα protein.
Background	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).