Cell Signaling Tra-2-54 (2j) Mouse mAb

Name: Cell Signaling Tra-2-54 (2j) Mouse mAb
SKU: CSIG-4747S
Price: 351.48 USD

List Price $351.48 Your Price $351.48

Cell Signaling Tra-2-54 (2j) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4747S

MFG.PART: 4747S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	80
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	TRA-2-54 (2J) Mouse mAb recognizes endogenous levels of total TRA-2-54 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with 2102Ep human embryonal carcinoma cells.
Background	The family of alkaline phosphatases in humans is comprised of four members: intestinal, placental, placental-like (germ cell type), and tissue nonspecific. The tissue nonspecific isozyme is also known as the liver/bone/kidney alkaline phosphatase due to its expression in these tissues. It is also highly expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells and is lost as these cells undergo differentiation (1,2). The TRA-2-54 (2J) Mouse mAb specifically detects this isozyme and does not detect the other family members, making it a useful tool for tracking the pluripotency status of ES and EC cells in culture.

Specifications