Cell Signaling Mlh1 (Es05) Mouse mAb

Name: Cell Signaling Mlh1 (Es05) Mouse mAb
SKU: CSIG-47954S
Price: 385.82 USD

List Price $385.82 Your Price $385.82

Cell Signaling Mlh1 (Es05) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-47954S

MFG.PART: 47954S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Source/Isotype	Mouse IgG1
Application/Dilution	{IHC Leica Bond: 1:100 - 1:400, Immunohistochemistry (Paraffin): 1:50 - 1:200}
Storage	Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.
Specificity/Sensitivity	MLH1 (ES05) Mouse mAb recognizes endogenous levels of MLH1 protein. Non-specific cytoplasmic staining was observed in pancreas by immunohistochemistry.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to 210 amino acids of human MLH1 protein.
Background	Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).

Specifications