Cell Signaling Mouse Anti-Rabbit Igg (Conformation Specific) (L27a9) mAb (Hrp Conjugate)

Name: Cell Signaling Mouse Anti-Rabbit Igg (Conformation Specific) (L27a9) mAb (Hrp Conjugate)
SKU: CSIG-5127S
Price: 244.42 USD

List Price $244.42 Your Price $244.42

Cell Signaling Mouse Anti-Rabbit Igg (Conformation Specific) (L27a9) mAb (Hrp Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5127S

MFG.PART: 5127S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	Rab
Source/Isotype	Mouse
Application/Dilution	{}
Storage	Supplied in 136 mm NaCl, 2.6 mm KCl, 12 mm sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. This antibody may cross react with denatured [light chain] IgG at high concentrations.
Species Reactivity	Rabbit
Source/Purification	Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Background	Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.