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Cell Signaling Trmt112 Antibody
List Price $320.17 Your Price $320.17
Cell Signaling Trmt112 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-57540S
MFG.PART: 57540S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 15 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | TRMT112 Antibody recognizes endogenous levels of total TRMT112 protein. This antibody detects a 20 kDa protein of unknown origin. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro108 of human TRMT112 protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | TRMT112 is an evolutionarily conserved 15 kDa protein that interacts with and stabilizes various methyltransferases involved in the methylation of tRNA, rRNA, and proteins. While most studies have been in yeast, TRMT112 has been shown to interact with human methyltransferases involved in translation and ribosome biogenesis, including TRMT11, ALKBH8, HEMK2a, WBSCR22, and METTL5 (1-5). TRMT112 is also widely expressed during embryogenesis (6).The TRMT11-TRMT112 complex deposits 2-methylguanosine at position 10 of tRNA, while ALKBH8-TRMT112 modifies a uridine at position 34 of the anti-codon loop (5,7) Additionally, TRMT112 plays a role in translation termination by interacting with N6AMT1, causing methylation of the release factor eRF1 to allow for stop codon recognition (8,9).TRMT112 also contributes to ribosome biogenesis by interacting with and stabilizing WBSCR22, a 18s rRNA methyltransferase, and METTL5, the 18s rRNA m6a-methyltranserase (3,4,10). |
| SKU | CSIG-57540S |
|---|---|
| Supplier Part Number | 57540S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |