Cell Signaling Cleaved Parp (Asp214) (D64e10) Xp Rabbit mAb (Sepharose Bead Conjugate)

Name: Cell Signaling Cleaved Parp (Asp214) (D64e10) Xp Rabbit mAb (Sepharose Bead Conjugate)
SKU: CSIG-5838S
Price: 497.93 USD

List Price $497.93 Your Price $497.93

Cell Signaling Cleaved Parp (Asp214) (D64e10) Xp Rabbit mAb (Sepharose Bead Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5838S

MFG.PART: 5838S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	400 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	89
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunoprecipitation: 1:20}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 of human PARP protein.
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Specifications