Cell Signaling Phospho-Src Family (Tyr416) (E6g4r) Rabbit mAb

Name: Cell Signaling Phospho-Src Family (Tyr416) (E6g4r) Rabbit mAb
SKU: CSIG-59548S
Price: 405.01 USD

List Price $405.01 Your Price $405.01

Cell Signaling Phospho-Src Family (Tyr416) (E6g4r) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-59548S

MFG.PART: 59548S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	60
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Src Family (Tyr416) (E6G4R) Rabbit mAb detects endogenous levels of Src, Fyn, Lyn, Lck, Hck, and Yes only when phosphorylated at Tyr416, Tyr420, Tyr397, Tyr394, Tyr411, and Tyr426, respectively. This antibody only minimally recognizes phosphorylated Blk and Fgr.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human Lyn protein.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Specifications