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Cell Signaling Phospho-Irf-3 (Ser396) (D6o1m) Rabbit mAb (BSA And Azide Free)
List Price $906.98 Your Price $906.98
Cell Signaling Phospho-Irf-3 (Ser396) (D6o1m) Rabbit mAb (BSA And Azide Free) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-61166SF
MFG.PART: 61166SF
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µg |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 45-55 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {} |
| Storage | Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #29047 |
| Specificity/Sensitivity | Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser396. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser396 of human IRF-3 protein. |
| Background | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double stranded RNA treatment. These events likely play a role in the activation of IRF-3 (8). |
| SKU | CSIG-61166SF |
|---|---|
| Supplier Part Number | 61166SF |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |