Cell Signaling Pyruvate Carboxylase Antibody

Name: Cell Signaling Pyruvate Carboxylase Antibody
SKU: CSIG-66470S
Price: 320.17 USD

List Price $320.17 Your Price $320.17

Cell Signaling Pyruvate Carboxylase Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-66470S

MFG.PART: 66470S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	130
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Pyruvate Carboxylase Antibody recognizes endogenous levels of total pyruvate carboxylase protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human pyruvate carboxylase protein. Antibodies are purified by peptide affinity chromatography.
Background	Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate in an anaplerotic pathway to replenish TCA cycle intermediates. It is also critical in regulating gluconeogenesis in the liver (1). Studies show that the increase of PC flux contributes to the induction of hepatic gluconeogenesis by glucagon (2). In addition, high-fat diet is shown to promote hepatic glucose uptake and induce PC activity in the liver (3). Loss of hepatic PC prevents diet-induced hyperglycemia and insulin resistance. Furthermore, this enzyme is also required for antioxidant defenses (1).

Specifications