Cell Signaling Cathepsin D (E179) Antibody

Name: Cell Signaling Cathepsin D (E179) Antibody
SKU: CSIG-69854S
Price: 317.14 USD

List Price $317.14 Your Price $317.14

Cell Signaling Cathepsin D (E179) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-69854S

MFG.PART: 69854S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	46, 43, 28
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Frozen): 1:50 - 1:200, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cathepsin D (E179) Antibody detects endogenous levels of preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu179 of mouse cathepsin D protein. Antibodies are purified by peptide affinity chromatography.
Background	Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9).

Specifications