Cell Signaling Phospho-Lyn (Tyr397)/Lck (Tyr394)/Hck (Tyr411)/Blk (Tyr389) (E5l3d) Rabbit mAb

Name: Cell Signaling Phospho-Lyn (Tyr397)/Lck (Tyr394)/Hck (Tyr411)/Blk (Tyr389) (E5l3d) Rabbit mAb
SKU: CSIG-70926S
Price: 419.15 USD

List Price $419.15 Your Price $419.15

Cell Signaling Phospho-Lyn (Tyr397)/Lck (Tyr394)/Hck (Tyr411)/Blk (Tyr389) (E5l3d) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-70926S

MFG.PART: 70926S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	60
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit mAb detects endogenous levels of LYN, LCK, HCK, and BLK only when phosphorylated at Tyr397, Tyr394, Tyr411, and Tyr389, respectively. It may cross react with overexpressed phosphorylated Src, YES, FYN, and FGR.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human LYN protein.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Specifications