Cell Signaling G4s Linker (E7o2v) Rabbit mAb

Name: Cell Signaling G4s Linker (E7o2v) Rabbit mAb
SKU: CSIG-71645T
Price: 213.11 USD

List Price $213.11 Your Price $213.11

Cell Signaling G4s Linker (E7o2v) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-71645T

MFG.PART: 71645T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	All
Sensitivity	Transfected Only
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Live): 1:50 - 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #63670.
Specificity/Sensitivity	G4S Linker (E7O2V) Rabbit mAb recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.
Species Reactivity	All Species Expected
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats.
Background	The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3 or 20-mer (G4S)4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).

Specifications