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Cell Signaling Cd16/Cd32 (2.4g2) Rat mAb (If Formulated)
List Price
$351.48
Your Price
$351.48
Cell Signaling Cd16/Cd32 (2.4g2) Rat mAb (If Formulated) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-80366S
MFG.PART:
80366S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | M |
| Sensitivity | Endogenous |
| Source/Isotype | Rat IgG2b |
| Application/Dilution | {Immunofluorescence (Frozen): 1:50 - 1:100, Immunofluorescence (Immunocytochemistry): 1:50 - 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. This product is stable for 36 months when stored at -20C. |
| Specificity/Sensitivity | CD16/CD32 (2.4G2) Rat mAb (IF Formulated) recognizes endogenous levels of total CD16/CD32 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD16 and CD32. Staining of both microglia and astrocytes was seen when staining was done in 5XFAD mouse brain. |
| Species Reactivity | Mouse |
| Source/Purification | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. |
| Background | CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).CD16 and CD32 are expressed on microglia within the brain (4). |
| SKU | CSIG-80366S |
|---|---|
| Supplier Part Number | 80366S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |