Cell Signaling Aml1 Antibody (Mouse Preferred)

Name: Cell Signaling Aml1 Antibody (Mouse Preferred)
SKU: CSIG-8229S
Price: 320.17 USD

List Price $320.17 Your Price $320.17

Cell Signaling Aml1 Antibody (Mouse Preferred) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-8229S

MFG.PART: 8229S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	55
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	AML1 Antibody detects endogenous levels of total AML1 protein. This antibody will recognize human AML1, however, we recommend AML1 (D33G6) XP® Rabbit mAb #4336 for detection of human AML1.
Species Reactivity	Human, Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val211 of human AML1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).