Cell Signaling Fen-1 (E5x2t) Rabbit mAb

Name: Cell Signaling Fen-1 (E5x2t) Rabbit mAb
SKU: CSIG-83104S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Fen-1 (E5x2t) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-83104S

MFG.PART: 83104S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	45
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:100 - 1:400, Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	FEN-1 (E5X2T) Rabbit mAb recognizes endogenous levels of total FEN-1 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length human FEN-1 protein.
Background	Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double-stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).

Specifications