Cell Signaling Beta-Catenin (D10a8) Rabbit mAbjugate)

Name: Cell Signaling Beta-Catenin (D10a8) Rabbit mAbjugate)
SKU: CSIG-88187S
Price: 469.65 USD

List Price $469.65 Your Price $469.65

Cell Signaling Beta-Catenin (D10a8) Rabbit mAbjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-88187S

MFG.PART: 88187S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H M R Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunofluorescence (Frozen): 1:50 - 1:200, Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600, Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	β-Catenin (D10A8) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total β-catenin protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein.
Background	β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Specifications