We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Cell Signaling P44/42 Mapk (Erk1/2) Antibody
List Price
$317.14
Your Price
$317.14
Cell Signaling P44/42 Mapk (Erk1/2) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-9102S
MFG.PART:
9102S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 200 µl |
| Reactivity | H M R Hm Mk Mi Z B Pg Sc |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 42, 44 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | p44/42 MAPK (Erk1/2) Antibody detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, this antibody recognizes p44 MAPK more readily than p42 MAPK. The antibody does not recognize either JNK/SAPK or p38 MAP kinase. |
| Species Reactivity | Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish, Bovine, Pig, S. cerevisiae |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence in the C-terminus of rat p44 MAP Kinase. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059. |
| SKU | CSIG-9102S |
|---|---|
| Supplier Part Number | 9102S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |